2017-8-11 16:0:32025-05-08 15:04:13
locus
BSU02390
BSU_02390
geneLength
1068
1071
outlinks
bsu
BSU02390
BSU_02390
Gene
Coordinates
259,016 → 260,086
259,016 260,086
The protein
Catalyzed reaction/ biological activity
l-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)
2-oxoglutarate + L-leucine --> 4-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
2-oxoglutarate + L-isoleucine --> (S)-3-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
2-oxoglutarate + L-valine --> 3-methyl-2-oxobutanoate + L-glutamate (according to UniProt)
The protein
Protein family
class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
[SW|Class-IV pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|YwaA]]
[[this]]
Biological materials
Mutant
MGNA-B939 (ybgE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1938 NBRP B. subtilis, Japan]
a ''ybgE'' mutant and a ''[[gene|bcd]] [[gene|ybgE]] [[gene|ywaA]]'' triple mutant are available in [SW|Linc Sonenshein]'s lab
MGNA-B939 (ybgE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1938 NBRP B. subtilis, Japan]
a ''ybgE'' mutant and a ''[[gene|bcd]] [[gene|ybgE]] [[gene|ywaA]]'' triple mutant are available in [SW|Linc Sonenshein]'s lab
BKE02390 ([[gene|ybgE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT
BKK02390 ([[gene|ybgE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT
Biological materials
lacZ fusion
pGP248 (in [[protein|pAC5]]), available in [SW|Jörg Stülke]'s lab
pGP248 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab
The protein
[SW|Cofactors]
PLP