SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-08 15:04:13

locus

BSU02390

BSU_02390

geneLength

1068

1071

outlinks

bsu

BSU02390

BSU_02390

Gene

Coordinates

259,016 → 260,086

259,016 260,086

The protein

Catalyzed reaction/ biological activity

l-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)

2-oxoglutarate + L-leucine --> 4-methyl-2-oxopentanoate + L-glutamate (according to UniProt)

2-oxoglutarate + L-isoleucine --> (S)-3-methyl-2-oxopentanoate + L-glutamate (according to UniProt)

2-oxoglutarate + L-valine --> 3-methyl-2-oxobutanoate + L-glutamate (according to UniProt)

The protein

Protein family

class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

[SW|Class-IV pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

The protein

Paralogous protein(s)

[[protein|YwaA]]

[[this]]

Biological materials

Mutant

MGNA-B939 (ybgE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1938 NBRP B. subtilis, Japan]

a ''ybgE'' mutant and a ''[[gene|bcd]] [[gene|ybgE]] [[gene|ywaA]]'' triple mutant are available in [SW|Linc Sonenshein]'s lab

MGNA-B939 (ybgE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1938 NBRP B. subtilis, Japan]

a ''ybgE'' mutant and a ''[[gene|bcd]] [[gene|ybgE]] [[gene|ywaA]]'' triple mutant are available in [SW|Linc Sonenshein]'s lab

BKE02390 ([[gene|ybgE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT

BKK02390 ([[gene|ybgE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT

Biological materials

lacZ fusion

pGP248 (in [[protein|pAC5]]), available in [SW|Jörg Stülke]'s lab

pGP248 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab

The protein

[SW|Cofactors]

PLP